A Validated RP-HPLC Method for Estimation of Glipizide in it?S Pure and Pharmaceutical Dosage Form (Tablets)

Mangesh Mantri* and S. Shanmukhappa

*Department of Chemistry, Bapuji Institute of Engineering and Technology, Davangere, Karnataka - 577 004 (India).

DOI : http://dx.doi.org/10.13005/msri/060131

ABSTRACT:

A simple, specific and precise high performance liquid chromatographic method has been developed for estimation of Glipizide in its pure and pharmaceutical dosage forms. A HPLC system consisting of a gradient pump, reverse phase C-18 analytical column, a variable UV-Visible detector set at 225 nm and an integrator was used. The mobile phase consisted of methanol and 0.115%w/v ammonium dihydrogen phosphate buffer and pumped at 1ml/min at ambient temperature. This RP-HPLC method was validated for its linearity, precision and accuracy. The calibration curve was linear in the range of 10 to 70 μg/ml).

KEYWORDS: Glipizide; HPLC; Hypurity C18 analytical column; placebo; tablets

Copy the following to cite this article: Mantri M, Shanmukhappa S. A Validated RP-HPLC Method for Estimation of Glipizide in it?S Pure and Pharmaceutical Dosage Form (Tablets). Mat.Sci.Res.India;6(1)

Copy the following to cite this URL: Mantri M, Shanmukhappa S. A Validated RP-HPLC Method for Estimation of Glipizide in it?S Pure and Pharmaceutical Dosage Form (Tablets). Mat.Sci.Res.India;6(1). Available from: http://www.materialsciencejournal.org/?p=3487

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